Coding

Part:BBa_K209490:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-01)

AarI B-D part, DOR-KIFC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2322
    Illegal PstI site found at 31
    Illegal PstI site found at 1279
    Illegal PstI site found at 1999
    Illegal PstI site found at 2032
    Illegal PstI site found at 2239
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2322
    Illegal NheI site found at 1785
    Illegal PstI site found at 31
    Illegal PstI site found at 1279
    Illegal PstI site found at 1999
    Illegal PstI site found at 2032
    Illegal PstI site found at 2239
    Illegal NotI site found at 1010
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2322
    Illegal BglII site found at 641
    Illegal BglII site found at 1757
    Illegal BamHI site found at 1141
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2322
    Illegal PstI site found at 31
    Illegal PstI site found at 1279
    Illegal PstI site found at 1999
    Illegal PstI site found at 2032
    Illegal PstI site found at 2239
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2322
    Illegal PstI site found at 31
    Illegal PstI site found at 1279
    Illegal PstI site found at 1999
    Illegal PstI site found at 2032
    Illegal PstI site found at 2239
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1229
    Illegal BsaI.rc site found at 1646
    Illegal BsaI.rc site found at 1768
    Illegal BsaI.rc site found at 1778


Design Notes

Cloned into pCR2.1.

Source

References